Determination of Equivalent Pore Radius for Human Red Cells by Osmotic Pressure Measurement
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چکیده
منابع مشابه
Determination of Equivalent Pore Radius for Human Red Cells by Osmotic Pressure Measurement
A new method has been developed to measure the equivalent pore radius in cellular membranes, and has been applied to human red cells. When red cells are suddenly introduced into a non-isosmolar concentration of non-lipid-soluble non-electrolyte molecules, water will enter or leave the cell. The rate of cell swelling or shrinking is determined and extrapolated to zero time to give the initial ra...
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The osmotic water permeability of human red cells has been reexamined with a stopped-flow device and a new perturbation technique. Small osmotic gradients are used to minimize the systematic error caused by nonlinearities in the relationship between cell volume and light scattering. Corrections are then made for residual systematic error. Our results show that the hydraulic conductivity, Lp, is...
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The aim of our experiments was to characterise and to validate the osmotic fragility test when applied to human blood samples with no significant alterations of osmotic fragility but with a differentiating shape of the haemolysis curve. All experiments were carried out on human erythrocytes taken from the Regional Centre of Blood Donation and Blood Therapy in Wrocław. The washed erythrocytes we...
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A new technique to determine the rate of water passage through the membrane of the human erythrocyte under an osmotic gradient has been developed. It utilizes a rapid mixing apparatus of the Hartridge-Roughton type which permits measurements at short intervals after the reaction has begun. This is coupled with a light-scattering device of new design which permits the determination of very small...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1960
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.44.1.1